primary antibody anti-ps6  (Cell Signaling Technology Inc)

Journal: NPJ Parkinson's Disease

Article Title: Impulsive-compulsive behaviours and striatal neuroactivity in mildly parkinsonian rats under D2/3 agonist and L-DOPA treatment

doi: 10.1038/s41531-025-00996-z

Figure Lengend Snippet: a pS6-immunopositive (pS6 + ) cell counts in the whole striatum under different treatments ( n = 69). Two-factor ANOVA was followed by Tukey’s post hoc test for the overall treatment effect. F(treatment) 3,61 = 8.9, p < 0.001; F(lesion) 1,61 = 0.9, p = 0.339; F(interaction) 3,61 = 1.7, p = 0.183. b pS6 + cell counts in the whole striatum and the denervated area in 6-OHDA-lesioned animals under different treatments ( n = 35). Two-factor repeated measurement ANOVA was followed by Tukey’s post hoc test for pairwise comparisons within one treatment or lesion type. F(treatment) 3,31 = 3.3, p = 0.033; F(area) 1,31 = 210.0, p < 0.001; F(animal) 31,31 = 15.9, p < 0.001; F(area x treatment) 3,31 = 8.4, p < 0.001. c Representative immunohistochemical stainings of pS6 + cells in the striatum developed using 3,3’ - diaminobenzidine (DAB). Shown are high magnification images of the intact and the lesioned area of one example animal per treatment. Scale bar: 30 μm. d Representative immunohistochemical stainings of pS6 + cell distributions in the striatum developed using DAB from one example animal per experimental group. Scale bar: 300 μm. Symbols of statistical significance: a = p < 0.05 vs Saline; b = p < 0.05 vs LD24; c = p < 0.05 vs R2.5; bracket = p < 0.05 for Total vs Lesioned area within the same treatment.

Article Snippet: For quantifying the extent of striatal dopaminergic denervation and counting of pS6 + cells, bright-field immunohistochemistry was performed using a primary antibody against TH (rabbit anti-TH, Pel-Freez P40101 , 1:1000) and phosphorylated pS6, respectively (monoclonal rabbit anti Ser235/236-phospho-S6, Cell Signaling #2211, 1:200).

Techniques: Immunohistochemical staining, Saline

Journal: NPJ Parkinson's Disease

Article Title: Impulsive-compulsive behaviours and striatal neuroactivity in mildly parkinsonian rats under D2/3 agonist and L-DOPA treatment

doi: 10.1038/s41531-025-00996-z

Figure Lengend Snippet: a –c Main principal components (PCs) identified in a principal component analysis of 2D histograms of pS6 + cell distributions in the striatum. Colour scale shows local variance, V max = maximal variance. Covariance is present in pixels with the same variance sign (positive: red, or negative: blue) and antivariance is present in pixels with opposite variance signs (red vs blue). a PC1. b PC2. c Inverted PC3. a’ –c’ Coefficients of main PCs as indices of the expression level of each covariance pattern for pS6 + cell distributions in different experimental groups. Two-factor ANOVAs were followed by Tukey’s post hoc test for pairwise comparisons within one treatment or lesion type; n(independent animals)=69, n(sections)=411. a’ Coefficient of PC1. F(treatment) 3,61 = 28.3, p < 0.001; F(lesion) 1,61 = 5.3, p = 0.024; F(interaction) 3,61 = 6.1, p = 0.001. b’ Coefficient of PC2. F(treatment) 3,61 = 1.6, p = 0.191; F(lesion) 1,61 = 62.3, p < 0.001; F(interaction) 3,61 = 21.7, p < 0.001. c’ Coefficient of inverted PC3. F(treatment) 3,61 = 10.0, p < 0.001; F(lesion) 1,61 = 2.1, p = 0.155; F(interaction) 3,61 = 7.3, p < 0.001. Symbols of statistical significance: a = p < 0.05 vs Saline within the same lesion type; b = p < 0.05 vs LD24 within the same lesion type; c = p < 0.05 vs R2.5 within the same lesion type; bracket = p < 0.05 for Sham vs 6-OHDA within the same treatment.

Article Snippet: For quantifying the extent of striatal dopaminergic denervation and counting of pS6 + cells, bright-field immunohistochemistry was performed using a primary antibody against TH (rabbit anti-TH, Pel-Freez P40101 , 1:1000) and phosphorylated pS6, respectively (monoclonal rabbit anti Ser235/236-phospho-S6, Cell Signaling #2211, 1:200).

Techniques: Expressing, Saline